TABLE 2.
Formation of ura+ recombinants depends on Cre recombinase and two loxP inserts
| Position of loxP insert
|
Recombination frequencya
|
||
|---|---|---|---|
| padh1-loxP | loxP-ura4 | −Cre | +Cre |
| arg1 | arg1 | <0.00022 | 79 ± 16.5 |
| arg1 | lys4 | <0.00017 | 4.5 ± 1.5 |
| None | arg1 | 0.16 ± 0.16 | |
| None | lys4 | 0.06 ± 0.02 | |
| None | arg7 | 0.03 ± 0.02 | |
| None | his1 | 0.05 ± 0.01 | |
| None | lys3 | <0.006 | |
Each value represents the average of three measurements.
Expressed as the number of ura+ recombinants/103 CPU.