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. 2007 Dec 13;27(1):111–121. doi: 10.1038/sj.emboj.7601955

Figure 3.

Figure 3

Fission yeast Wapl regulates cohesin dynamics in G1. (A) Cells were arrested in G1 at 20°C by Res1 C-terminal overexpression and then shifted to 37°C for 2 h. Rad21-GFP association with chromatin was monitored on chromosome spreads by immunofluorescence using anti-GFP antibodies. Scale bar, 5 μm. (B) Rad21-GFP fluorescence intensity was measured for 50–100 nuclei per sample. The error bars show the confidence interval of the mean with α=0.05. (C) Strains bearing rec8-GFP were arrested in G1 by nitrogen starvation at 25°C to induce Rec8-GFP accumulation at centromeres and then shifted to 37°C for 90 min. Samples were taken every 30 min and examined for Rec8-GFP fluorescence. Scale bar, 5 μm. (D) Proportion of cells with a dot of Rec8-GFP after inactivation of Mis4. More than 150 cells were examined for each sample. (E) Quantification of Rec8-GFP fluorescence before and 90 min after the temperature shift. More than 150 cells were analyzed for each sample. The error bars show the confidence interval of the mean with α=0.05. (F) Relative Rec8-GFP fluorescence intensity after shift to 37°C. Rec8-GFP fluorescence for each strain is normalized to its value at the time of temperature shift.