Figure 6.

Effect of RNAi on free minicircle replication intermediates. Total DNA was fractionated on an agarose gel with TBE buffer containing 1 μg/ml ethidium bromide (Wang and Englund, 2001). (A) Autoradiograph of gel probed for minicircles and trypanosome hexose transporter (THT1) as loading control. N/G, nicked and gapped free minicircles that are the products of replication; CC, covalently closed free minicircles that are the substrates for replication. (B) Quantitation of free minicircles by phosphorimaging with values corrected for loading control. Values are the mean (±standard deviation) of two experiments, one of which is shown in panel A.