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. 1999 Jun 22;96(13):7232–7237. doi: 10.1073/pnas.96.13.7232

Figure 2.

Figure 2

Refolding kinetics of α-helix formation monitored by stopped-flow CD at 222 nm or 226 nm. Data were fitted to the equations θ = A (1 − e−kt) + θ(U) or
graphic file with name M1.gif
(27). (A) AK16. (B) AQ28. (C) Poly(Lys). (D) Poly(Glu). Data were fitted to the three-state U → I → F mechanism by using kaleidagraph (Synergy Software, Reading, PA). Parameters fitted: U->I rate constant (k1), I->F rate constant (k2), θ226 (U), θ226 (I), and θ226 (F). Errors are SE after kaleidagraph fitting.