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Reactivity of an affinity-purified CL154C-reactive IgG with cardiolipin. The unfractionated IgG, eluate (CL154C-reactive), and the pass IgG fractions, as well as control polyclonal human IgG were analyzed at the indicated concentrations (1.25–5 μg/ml) in duplicate. Bound IgG was measured and expressed in optical density (OD). Results are representative of 1 of 2 experiments.
