Table 1.
Peptide sequences and characterization of recurrent clones enriched from 17 phage-display peptide libraries by CL15*
| Panning, sequence | OD | Frequency | Clone designation |
|---|---|---|---|
| Pooled libraries | |||
| APVCV WERLS PYRER CV | 0.45 | 5/5 | CL151 |
| SCPAN TYACM | 0.47 | 5/5 | CL152 |
| VTVTY N | 0.45 | 3/4 | CL153 |
| EPTPS SFVHY RLNAA† | 0.49 | 1/4 | |
| None | 0.11 | NA | f88-4 vector |
| Individual library | |||
| HWDPF SLSAY FP | 0.51 | 5/8‡ | CL154 |
| None | 0.06 | NA | M13 |
Seventeen libraries were screened by CL15 in 6 pools of 2–3 libraries each. In addition, 2 of 17 libraries were screened individually. After 4 rounds of panning, 4–5 clones from each pool were isolated and sequenced. CL151 was from pool 2 (library X15CX), CL152 from pool 4 (library LX6), CL153 from pool 5 (library X6), and CL154 from the New England Biolabs linear 12-mer library. OD denotes the mean optical density of all clones with the indicated insert, as determined by the reversed phage enzyme-linked immunosorbent assay format. Frequency denotes the number of phage clones with identical inserts.
NA = not applicable.
This sequence occurred only one time, and thus was not studied and not assigned a name.
After 4 rounds of panning, 10 clones were isolated and sequenced.