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HPLC fractionation on the Supelcosil C8-DB column of the dark-color-inducing neuropeptide digested with pyroglutamate aminopeptidase (A) and of the natural peptide (B) (for column conditions, see text). The hardly noticeable peak of the parent peptide at 18 min in A indicated that nearly all of the parent peptide had been digested by the pyroglutamine aminopeptidase. AUFS, absorption units full scale.
