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. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: FEBS Lett. 2007 Nov 20;581(29):5611–5616. doi: 10.1016/j.febslet.2007.11.010

Fig. 3. Impact of 5’-phosphate on Dicer binding affinity for ssRNAs and siRNAs.

Fig. 3

Dicer-ssRNA binding reactions were performed in the presence of 100-fold excess unlabeled ssRNA (lanes 3 and 5) or siRNA (lanes 4 and 6). In lanes 3 and 4, competition was performed with 5’-hydroxyl RNAs. In lanes 5 and 6, competition was performed with 5’-phosphate RNAs. The arrow indicates the position of the Dicer-ssRNA complex.