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Biosynthesis of the benzoxazolinate moiety of C-1027. (A) Schematic of the C-1027 genetic locus with the eight putative ORFs identified by using BLAST analysis, including the sgcD/sgcG subcluster. (B) The enzymatic steps for the conversion of chorismate to the benzoxazolinate moiety. Highlighted in bold are the two reactions characterized in this study. Glu, glutamate; α-KG, α-ketoglutarate; FMN, flavin-mononucleotide; other abbreviations are defined in Fig. 2.
