Skip to main content
. 2008 Jan 8;105(2):494–499. doi: 10.1073/pnas.0708750105

Fig. 4.

Fig. 4.

HPLC analysis of SgcD and SgcG reactions. (A) HPLC profiles of chorismate incubated with boiled SgcD (I), chorismate incubated with SgcD (II), the purified SgcD product ADIC incubated with E. coli ASI (III), and anthranilate standard (IV). (B) HPLC profiles of authentic ADIC (I), ADIC incubated with SgcG (II), ADIC incubated with ASI (III), and ADIC incubated with SgcG and spiked with authentic anthranilate (IV). Open diamonds, chorismate; filled diamonds, ADIC; open inverted triangles, anthranilate; open circles, FMN; filled circles, OPA. Anth., anthranilate; other abbreviations are defined in Fig. 2.