Fig. 1.

Positions of myc-tag insertion in the murine P14 TCR. (A and B) The 10-aa myc-tag sequence was incorporated into the sequence of the TCRα-chain (A) or the TCRβ-chain (B). Four constructs were generated, in which one or two myc-tags were inserted (marked with a “1”). Numbers indicate the amino acid position behind which the tag was introduced. In five constructs, the sequences of the original TCR were substituted with one or two myc-tag sequences (marked with a “2”). Here, numbers indicate the first and last amino acid position of the original sequence that was replaced. The position selected for the modification of other human and murine TCRs is marked by an asterisk. S, signal peptide; V, variable region; D, diversity region; J, joining region; C, constant region.