Fig. 3.

TCRmyc-transduced T cells can be depleted in vitro and in vivo with a myc-specific antibody. (A) The 58 cells were transduced with OT-I/TCRwt or OT-I/TCRmyc and were enriched with vβ5-specific antibodies. For depletion, cells were incubated with a myc-specific antibody and rabbit complement factors. 7-AAD was used to discriminate between living and dead cells. Numbers indicate the calculated specific lysis. Results show data from one of two independent experiments with comparable results. (B) Splenocytes of B6 mice were transduced with either OT-I/TCRwt or OT-I/TCRmyc. The 5 × 10⁶ TCR-transduced cells were adoptively transferred i.v. into Rag-1^−/− recipients. After 13 days, blood was stained for CD8- and myc-positive cells. One group of mice received 500 μg of a myc-specific antibody i.p. for depletion. One day after antibody injection, blood samples were collected and stained with CD8- and myc-specific antibodies for the TCRmyc construct or with CD8-, vα2- and vβ5-specific antibodies for the TCRwt construct. Stainings show cells gated on CD8 expression and represent one of two treated animals.