Fig. 3.
Mm induces secretion of lysosomes from macrophages. (A) Secretion of β-hexosaminidase by BMDM was measured 2 h after treatment with buffer (NB, no bacteria), Mm (MOI of 1, 10, or 50), or latex beads (beads, 50:1). (B) Viability of BMDM was measured 2 h after addition of inoculum. Cell viability is displayed as Absinfected/Absuninfected. (C) Secretion of lysosomal enzyme β-glucuronidase was measured 2 h after treatment of WT and HexB−/− BMDM with buffer (uninfected) or Mm (Mm). (D) Secretion of β-hexosaminidase was measured in Mm-infected WT or HexB−/− BMDM. β-Hexosaminidase activity is displayed in arbitrary units (A.U.). All assays were done in triplicate.