Skip to main content
. 2008 Jan 8;105(2):740–745. doi: 10.1073/pnas.0703842105

Fig. 2.

Fig. 2.

Pep4 and NEP1–40 prevent p75NTR-dependent motor neuron death. (A) Survival rates of cultured rat embryonic (E15) motor neurons treated for 24 h with 100 nM Pep4 or NEP1–40 in the presence (GDNF) or absence (none) of GDNF. Pep4 and NEP1–40 did not modify motor neuron survival in any condition (P > 0.05; n = 5 independent experiments). (B) Cultures were treated for 24 h with 100 nM Pep4 or NEP1–40 in the presence of NGF or NGF plus NOC-18 (NO generator) (*, P < 0.05 vs. control; #, P < 0.05 vs. NGF+NO alone; n = 5 independent experiments). (C) Rat embryonic (E15) motor neurons were cocultured on rat astrocyte monolayers and treated for 24 h with 100 nM Pep4 or NEP1–40 in the presence (NGF) or absence (none) of NGF (*, P < 0.05 vs. control; #, P < 0.05 vs. NGF; n = 3 independent experiments). (D) Cultures were treated for 24 h with 100 nM Pep4 or NEP1–40 and lumbar spinal cord lysates from symptomatic SOD1(G93A) or nontransgenic (WT) littermates in the presence of NOC-18 (NO generator) (*, P < 0.05 vs. G93A; #, P < 0.05 vs. G93A+NO; n = 3 independent experiments). (B–D) GDNF was present in all experimental conditions.