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. 2008 Jan 4;105(2):763–768. doi: 10.1073/pnas.0711053105

Fig. 4.

Fig. 4.

Unbiased estimation of nigral TH+ cells in SNc of animals injected with wt or mutant human α-syn. Tissue slices were labeled with antibody to TH (A–C) or stained with cresyl violet (D) as described in Materials and Methods and the percentage of surviving cells was calculated by comparison with the uninjected side in the same animal. The graphs display a comparison of wt injected animals compared with GFP as a function of time postinjection (A), and a comparison of the wt, 129A, and 129D animals compared with GFP at 4 wk (B) and 8 wk (C) postinjection. The GFP control in A–C is the same and includes 4-, 8-, and 26-wk samples as these did not vary significantly. The wt 4-wk and 8-wk groups in B and C are the same as in A and are repeated to facilitate comparison. (D) Eight-week samples stained with cresyl violet. Group ANOVA statistics were for (A) F[3,34] = 5.973, P = 0.002; (B) F[3,32] = 13.53, P = 0.001; (C) F[3, 29] = 9.22, P = 0.0002; (D) F[3,15] = 9.431, P = 0.001. Tukey's post hoc results are indicated as *, **, *** = P < 0.05, 0.01, and 0.001, respectively vs. GFP; #, ##, ### = P < 0.05, 0.01, and 0.001 vs. 129D; ++ = P < 0.01 vs. wt 4 wk.