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The unfolding free energies for residues in CP13*. (A) C-terminal residues (green) are dramatically more stable than N-terminal residues (blue). (B) The C-terminal subdomain is more stable than the N-terminal subdomain. Spheres represent residues that were probes in the NSHX experiment. (C) Boltzmann diagram of the native, PUF, and unfolded conformations for CP13* and WT*.
