Figure 2.

Multiple sequence alignment among cC273, hC273, yC273, and Af2198. The three Prp8 sequences are aligned using MultAlin (Corpet 1988). Af2198 is aligned with cC273 based on structural superimposition. Secondary structures as they are observed in the crystal structures of cC273 and Af2198 are labeled on top of the cC273 sequence and the bottom of the Af2198 sequence, respectively. 3₁₀ helices are not differentiated from α helices and are both labeled as helices with prefix α for simplicity. Gray represents residues that are disordered in the crystal structure. Gray bars highlight residues in the JAMM motif. Boxed and underlined residues are missense and frameshift RP13 mutants, respectively.