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GST-yC273 can pull down in vitro-translated ySnu114 (residues 618–1008). GST alone and GST-yC273 were expressed in E. coli, purified with glutathione resin, incubated with in vitro-translated ySnu114 (35S-labeled), washed, and separated by SDS-PAGE. The polyacrylamide gel was stained with Coomassie Blue to show equal loading of GST and GST-yC273 (bottom). The gel was dried, and bound ySnu114 was visualized by autoradiography (top).
