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. 2007 Jun;16(6):1165–1175. doi: 10.1110/ps.062623707

Figure 5.

Figure 5.

(A) Separation of bis-ANS-labeled peptide by HPLC. Trypsin-digested bis-ANS-labeled TF was separated on a C18 reverse-phase column using a gradient of 0.1% TFA in water and 0.1% TFA in acetonitrile. The eluate was monitored at 220 nm (solid line) for general peptides and 385 nm (dashed line) for bis-ANS-labeled peptides. (B) MALDI-TOF mass spectrum of bis-ANS-labeled peptide. The MS spectrum of the labeled peptide (m/z 855.30) was obtained from the MALDI-TOF MS analysis of 100 pmol-labeled peptide.