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. Author manuscript; available in PMC: 2008 Jan 18.
Published in final edited form as: J Immunol. 2006 Aug 1;177(3):1698–1707. doi: 10.4049/jimmunol.177.3.1698

FIGURE 5.

FIGURE 5

Expression of mutant La protein in transgenic mice. (a) Extracts from mutant La transgenic mice (muLa-tg; M1,M2) and non-transgenic mice (non-tg) were prepared from spleen tissues and analyzed by SDS-PAGE/immunoblotting with the anti-La mAb SW5 which recognizes the N-domain of human La. The extracts were normalized with anti-actin antibodies. (b) Extracts from different tissues of mutant La transgenic mice (muLa-tg) were prepared from thymus, spleen, liver, brain, kidney and intestine, immunoprecipitated with the anti-La mAb SW5 and analyzed by SDS-PAGE/immunoblotting with the anti-La mAb SW5 which recognizes the N-domain of human La and therefore also reacts with mutant human La (muLa). (c) Tissues from mice transgenic for mutant La (muLa-tg) or native La (nLa-tg) or from non-transgenic mice (non-tg) were stained with the human anti-La specific mAb SW5 (see Materials and Methods). Due to the lack of the NLS, mutant La (muLa-tg, SW5) is in the cytoplasm while native La (nLa-tg, SW5) is in the nucleus. SW5 does not stain tissues from non-transgenic mice irrespective of the background (FVB/N or B6). The negative controls (negative) were also not stained.