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The effect of pH on phytase activity of PhyAsr and the Asp223Asn mutant. Standard phosphatase assays were run over a pH range of 3.5–7. The overlapping buffer systems used were: 50 mM formate (pH 3.5–4), 50 mM sodium acetate (pH 4–6), and 50 mM imidazole (pH 6–7). The data are mean values ± standard deviation of three separate experiments. (Solid circles) Wild type; (open circles) D223N.
