High-performance ion-pair chromatography (HPIC) analysis of the hydrolysis products of IPPs by PhyAsr. (A) Reference sample containing a mixture of IPP standards (≤6 phosphates). The source and identity of the unmarked standard peaks is indicated in Skoglund et al. (1998). Peaks: (1) D/L-Ins(1,2,4,5,6)P5; (2) Ins(2,4,5,6)P4; (3) D/L-Ins(1,2,5,6)P4; (4) D/L-Ins(1,2,4,6)P4; (5) D/L-Ins(1,4,5)P3; D/L-Ins(2,4,5)P3; (6) D/L-Ins(1,2,6)P3, Ins(1,2,3)P3; (7) D/L-Ins(1,2,4)P3; (8) D/L-Ins(2,4)P2; (9) D/L-Ins(1,2)P2, Ins(2,5)P2, D/L-Ins(4,5)P2. (B) PhyAsr incubated with Ins P6 for 30 min. (C) PhyAsr incubated with Ins P6 for 90 min. Peaks representative of major pathway products are labeled accordingly.