Table 1.
Laboratory methods for assay of bone-turnover markers, lipids, and estrogens
| Parameter | Method | Manufacturer |
| Bone resorption | ||
| Adjusted U-CTx | Enzyme immunoassay test using the CrossLaps® ELISA kit | Nordic Bioscience Diagnostics A/S, Herlev, Denmark |
| S-CTx | Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer | Roche Diagnostics, Basel Switzerland |
| Adjusted U-NTx | Competitive immunoassay technique using the NTx reagent pack kit | Ortho-Clinical Diagnostics, Amersham, UK |
| Bone formation | ||
| BAP | Ostase Assay using Access Immunoassay System | Beckman Coulter, Villepinte, France |
| PINP | Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer | Roche Diagnostics |
| Lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) | Validated HPLC–RIA technique using Bayer Advia Reagent Packs. Assayed on an ADVIA® 1650 chemistry system analyzer | Bayer Diagnostics, Bayer Inc., Tarrytown, NY, USA |
| Estrogen (E1, E2, and E1S) | Validated HPLC–RIA technique | Aster-Cephac, Paris, France |
BAP, bone alkaline phosphatase; E1, estrone; E1S, estrone sulfate; E2, estradiol; ELISA, enzyme-linked immunosorbent assay; HDL, high-density lipoprotein; HPLC–RIA, high-performance liquid chromatography radioimmunoassay; LDL, low-density lipoprotein; PINP, procollagen type I N-terminal propeptide; S-CTx, serum C-terminal telopeptide of type I collagen; U-CTx, urine-adjusted urinary C-terminal telopeptide of type I collagen; U-NTx, urine-adjusted urinary N-terminal telopeptide of type I collagen.