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Fluorescence spectroscopy. Samples of N-62 StAR at pH 8.3, 4.0, 3.5, and 3.0 were excited at 295 nm in the absence or presence of 2.0, 4.0, and 6.0 M urea. Only the data without urea and with 6.0 M urea at pH 4.0, 3.5, and 3.0 are shown. The pH 8.3 and 4.0 data in the absence of urea were equivalent. The effects of 2.0 and 4.0 M urea were intermediate between those of 0.0 and 6.0 M urea. The wavelengths of the emission maxima in each condition are given in Table 2.
