Figure 2.

Secretory activity of chromaffin cells expressing GFP–S-25 constructs. (A) Amperometric traces representative from experiments performed in cells expressing full length SNAP-25 (GFP–S-25) and a truncated form (GFP–S-25 Δ9). Shown are also traces obtained from control nontransfected cells (CONTROL) present in the same culture plates that transfected cells. (B) Cumulative event analysis. Conditions were as described in A, after building of event cumulative responses for individual cells. The average secretion was obtained from control nontransfected (n = 14, continuous line), GFP–S-25 (n = 12, pointed line), and GFP–S-25 Δ9-expressing cells (n = 13, dashed line).