Figure 2.

Northern analysis of expression of AtPCS1 transcript in roots and shoots of Arabidopsis. Total RNA was extracted from 21-day-old Arabidopsis seedlings and hybridized with ³²P-labeled random-primed AtPCS1 cDNA. Ten μg of RNA was loaded on each lane. The 1.7-kb bands shown were the only ³²P-labeled bands detected after probing with AtPCS1. Also shown are the results of hybridizing the same blots with a probe directed against 18S rRNA to verify that similar amounts of RNA had been loaded on each lane.