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. 1999 Jun 8;96(12):7110–7115. doi: 10.1073/pnas.96.12.7110

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Copyright © 1999, The National Academy of Sciences

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Gel-filtration chromatography of soluble fractions extracted from DTY167/pYES3-AtPCS1∷FLAG (O) and DTY167/pYES3 cells (●) after growth in media containing ¹⁰⁹CdCl₂ (main figure). Direct comparison of the ¹⁰⁹Cd-radioactivity profile of peak 1 from the soluble fraction extracted from DTY167/ pYES3-AtPCS1∷FLAG cells (O) with the ¹⁰⁹Cd radioactivity profile obtained after Superose-6 chromatography of immunoaffinity-purified AtPCS1-FLAG after equilibrium dialysis against 5 μM ¹⁰⁹CdCl₂ (histogram) (Peak 1). AtPCS1-FLAG was detected by SDS/PAGE and Western analysis of 20 μl aliquots of pooled pairs of fractions from chromatography of the DTY167/pYES3AtPCS1∷FLAG extracts.