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Purification of AtPCS1-FLAG by immunoaffinity chromatography of the soluble fraction from DTY167/pYES4-AtPCS1∷ FLAG cells on an anti-FLAG M2 immunoaffinity column. Silver-stained SDS gel of soluble fraction before (5 μg protein) (A) and after (1 μg protein) (B) purification of AtPCS1-FLAG. Western analysis of FLAG epitope before (25 μg protein) (C) and after (1 μg protein) (D) purification of AtPCS1-FLAG. Positions of molecular-weight markers (Mr × 103) are indicated.
