Skip to main content
. 1999 Jun 22;96(13):7265–7270. doi: 10.1073/pnas.96.13.7265

Figure 3.

Figure 3

Expansion of hematopoietic cells on the fetal hepatic culture and its inhibition by OSM. One-thousand Lin−Sca-1+c-Kit+ cells were incubated on fetal hepatic culture in the presence of SCF with or without either IL-6 (A) or Epo (B) for 10 days, and the floating hematopoietic cells were counted. Note that the presence of OSM strongly suppressed expansion of hematopoietic cells induced either by SCF alone or a combination of SCF/IL-6 or SCF/Epo. Each histogram represents the mean value of triplicate experiments ± SD of the absolute number of cells generated in 60-mm-diameter dishes. (C) Percentages of TER119-positive cells generated in the presence of SCF alone (Left), SCF/Epo (Center), or SCF/Epo/OSM (Right). PI; Propidium iodide used to eliminate fluorescence derived from dead cells.