Table 2.
Erythroid colony-forming activities
| Culture conditions | cfu-e/104 cells | cfu-e/dish |
|---|---|---|
| SCF | 3.0 ± 0.8 | 534 ± 138 |
| SCF/OSM | 3.3 ± 0.4 | 383 ± 46 |
| SCF/EPO | 94.0 ± 7.0 | 31960 ± 2380 |
| SCF/EPO/OSM | 23.7 ± 3.9 | 1824 ± 300 |
| SCF/EPO (+OSM)* | 98.0 ± 1.4 | - |
| B.M. | 57.7 ± 4.1 | - |
| B.M. (+OSM)* | 57.0 ± 1.6 | - |
Shown are cfu-e activities of hematopoietic cells generated on the fetal hepatic culture. The number of erythroid progenitors in floating hematopoietic cells generated in culture conditions as indicated were determined by the methylcellulose colony formation assay. Cells were incubated for 3 days in the presence of Epo, and the number of erythroid colonies were counted by microscopic observation. As a control, bone marrow-derived hematopoietic cells (B.M.) were similarly tested. Data represent the mean values of triplicate experiments ± SD.
*
In these cases, OSM (100 ng/ml) was added during colony assays to exclude the possibility that OSM directly inhibited erythropoiesis.