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Localization of the α1A-adrenergic receptor by subcellular fractionation. Rat renal outer cortical tissue slices were incubated with or without NPY (10−6 M) plus phenylephrine (10−6 M) for 15 min, subjected to subcellular fractionation, and immunoblotted for α1A-adrenergic receptors. For experimental details, see legend to Fig. 2.
