Table 1.
Cell division in differentiated adipocytes
Cell line | Frequency of mitotic figures; cell counts (%)
|
|
---|---|---|
Experiment I | Experiment II | |
3T3L1 parental | 1/3,635 (<0.03) | 0/3,279 (<0.03) |
3T3L1 E7HPV-16 | 63/4,361 (1.4) | 51/3,692 (1.38) |
3T3L1 E7Ala31,32 | 12/5,467 (0.22) | 10/4,792 (0.21) |
3T3L1 cultures, as indicated in the left column, were differentiated for 10 days, and several thousand differentiated cells, as indicated, were microscopically inspected for mitotic figures. Enumeration shows absolute numbers of mitotic cells out of absolute numbers of all differentiated cells evaluated. Percentages of differentiated mitotic cells are given in parentheses for easier comparison. Two independent experiments are shown.