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Time-course effect of BMP-7 on E and P production by granulosa cells. Granulosa cells (5 × 104 viable cells per well/200 μl) were cultured for 48 h in serum-free medium containing androstenedione (1 μM) and FSH (3 ng/ml) in the absence (□) or presence of BMP-7 (30 ng/ml, ■). After culture, E and P levels in the conditioned medium were measured by RIA.
