Figure 2.

Characterization of actin defects in cells lacking each core Arp2/3 complex subunit. (A) Phalloidin staining of cells lacking each Arp2/3 complex subunit: wild-type (RLY1), Δarc15 (RLY570), Δarc18 (RLY571), Δarc19 (RLY572), Δarc35 (RLY573), Δarp2 (RLY574), Δarp3 (RLY739). Arrow points to actin cables and arrowheads to cytokinetic actin rings. (B) Localization of Cof1p, Cap2p, or Abp1p in wild type (RLY1) or Δarp2 (RLY574) cells. An outline has been drawn to show the profile of the Δarp2 cell stained with anti-Cof1p. (Bar = 10 μm.)