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. 1999 Jun 22;96(13):7300–7305. doi: 10.1073/pnas.96.13.7300

Figure 2.

Figure 2

PI 3-kinase, which is required for survival, interacts with ezrin. (A) LY294002 induces apoptosis of LLC-PK1 cells in the tubulogenesis assay. Mean ± SD of an experiment performed in triplicate is plotted. LY294002 concentration is given in μM. (B) Coimmunoprecipitation of either wild-type or Y353F ezrin with the p85 subunit of PI 3-kinase. Lysates from P, E, or F clones were immunoprecipitated with p85 affinity-purified antibodies or rabbit IgG. The immunoprecipitates were analyzed with P5D4 mAb (Tag) or polyclonal anti p85 antibodies. (C) p85 binding to the amino-terminal domain of ezrin. A lysate from placenta was incubated with immobilized GST, GST–ezrin1–309 (GST-N), or GST–ezrin310–585 (GST-C). After washing, bound material was analyzed with polyclonal anti-p85 antibodies.