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Sucrose gradient sedimentation of HeLa cell Cdc20 and aurora2/Aik. Cell lysates from a synchronized population of HeLa G2-phase cells were separated by sedimentation on 15–50% sucrose gradients. Fractions were analyzed by Western blotting as indicated. T is a sample of the total extract loaded on the gradient, and numbers indicate gradient fractions (top to bottom). Molecular weight markers are as in Fig. 3.
