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. 1999 Jun 22;96(13):7312–7317. doi: 10.1073/pnas.96.13.7312

Figure 1.

Figure 1

The inhibitors of IκB phosphorylation TPCK and BAY 11–7082 block NF-κB activation and induce apoptosis in T. parva-transformed T cells. (A) TPCK and BAY 11–7082 inhibit IκBα phosphorylation Whole-cell protein extracts were prepared from cells treated with TPCK (25 μM) or BAY 11–7082 (50 μM) for different lengths of time, and immunoblot analysis was carried out by using anti-IκBα antibodies. (B) T. parva-transformed cells were transfected with the −121/+232 HIV-CAT construct and cultured in the presence or absence of TPCK and CAT activity determined after 16 h. Error bars represent the SD for three independent experiments. (C) Logarithmically growing T. parva-infected T cells were treated with TPCK (25 μM) or BAY 11–7082 (50 μM) for 5 h, and annexin-V-FITC binding was analyzed by FACScan.