Figure 3.

BSN-CM contains unique soluble factor(s) for branching morphogenesis of the isolated UB. The UBs were cultured in the presence of the key growth factor (GDNF; see Fig. 5) but with different cell-conditioned media. (A) 3T3 fibroblast cell-conditioned medium. (B) Immortalized UB cell-conditioned medium. (C) mIMCD cell-conditioned medium. (D) BSN cell-conditioned medium. After culture, the UBs were fixed and processed for DB lectin staining. Only BSN-CM could promote branching morphogenesis of the isolated UB.