Table 3.
Analysis of REP1 expression in single cells
| Sample | No. cells | Allele detection, %
|
||
|---|---|---|---|---|
| Xa only | Xi only | Xa and Xi | ||
| Model A | 37 | 22 | 27 | 51 |
| Model B | 41 | 47 | 9 | 44 |
| Model C | 39 | 72 | 15 | 13 |
| Line 51 | 127 | 30 | 9 | 61 |
Single-cell RT–PCR detection of REP1. For each sample, the percentage of successfully amplified cells (or, for the control experiments, the number of wells containing the RNA equivalent of a single cell) that showed expression of one or the other allele (or both) is indicated. For the controls, homozygous cell lines were mixed to mimic Xi expression that was: equivalent to Xa expression in all cells (model A); reduced to 25% of Xa expression levels in all cells (model B); or equivalent to Xa expression, but only in one-third of cells (model C). Independent experiments gave similar results.