Table 1.

p53 gene mutations detected by direct dideoxynucleotide sequencing and not detected by the GeneChip p53 assay

Sample	p53 gene mutation∗	p53 GeneChip score†	Confirmed‡
741	1-bp deletion at 154	0	Yes
792	1-bp deletion at 154	6§	Yes
826	TGC → GGC at 176	12§	Yes
829	11-bp deletion at 132	0	ND
856	GGC → GTC at 154	0	Yes
970	GGA → TGA at 199	0	Yes
1,044	1-bp deletion at 299	0/5§	Yes
1,140	CGC to GTC at 158	0/0	Yes
1,217	complex rearrangement, exon 6	0/0	ND
1,217	ATC → ACC at 255	0	Yes
1,263	t → a, intron 8	0	ND

^*p53 mutation gene mutation at given codon determined by direct dideoxynucleotide sequencing. 

^† Samples 1,044, 1,140, and 1,217 were analyzed twice from two different PCR amplifications. 

^‡Mutation confirmed by hybridization of mutant specific oligonucleotide probe to PCR products from tumor DNA and not to normal DNA. ND, not done. 

^§Scores of 12 or less are not considered definite mutations (see Methods).