Table 1.
TGase activity is increased in HD brain
| Source of brain sample | TGase activity, cpm
|
|||
|---|---|---|---|---|
| + Casein | + Casein + MDC(n = 2) | − Casein | − Casein + MDC(n = 2) | |
| HD cortex | 14,888 ± 2,863* (n = 3) | 94 ± 18 | 464 ± 104 | 81 ± 17 |
| HD cerebellum | 11,221 ± 2,426† (n = 3) | 186 ± 57 | 477 ± 61 | 58 ± 13 |
| Control cortex | 6,697 ± 1,410‡ (n = 5) | 63 ± 10 | 607 ± 167 | 151 ± 39 |
| Control cerebellum | 2,606 ± 719 (n = 3) | 83 ± 14 | 145 ± 50 | 117 ± 30 |
| HD cortical nuclear extract | 6,368 ± 764§ (n = 3) | ND | 153 ± 23 | ND |
| Control cortical nuclear extract | 2,357 ± 226 (n = 4) | ND | 189 ± 32 | ND |
TGase activities (mean ± SEM) were measured as described in the text; n, number of human patients analyzed. By unpaired Student’s t test: ∗, P < 0.009 comparing HD to control cortex; †, P < 0.001 comparing HD to control cerebellum; ‡, P < 0.04 comparing control cortex to control cerebellum; §, P < 0.0001 comparing HD to control cortical nuclear extract. ND, not done.