Figure 2.

Mutations in unc-64 and unc-31 do not enhance the longevity of daf-2 mutants. Animals were grown at 15° to allow development past the dauer stage and then were shifted to 20°. unc-64; unc-31 had a significantly longer life span than either unc-64 (P = 0.0002) or unc-31 (P = 0.0005). The daf-2 unc-64 double mutant and daf-2 were not significantly different (P = 0.5139). The maximum life span of daf-2 unc-64 was extended to >100 days by a single animal. We have not investigated whether this has any possible significance. This figure uses the same data set as Table 2.