Table 2.

Mutations in unc-64 and unc-31 do not enhance the dauer and life span phenotypes of daf-2 mutants

Genotype	Percent of dauers at 15°C*	Days lived at 20°C†
N2 (wild-type)	0	24  ± 7 (20)
unc-63(e246)	1	33  ± 14 (27)
unc-31(e928)	0	35  ± 8 (29)
daf-2(e1370)	0	57  ± 9 (31)
daf-2(e1370) unc-64(e246)	1	59  ± 13 (37)
daf-2(e1370); unc-31(e928)	1	47  ± 9‡ (62)
unc-64(e246); unc-31(e928)	67	43  ± 11 (36)

^*Results are the mean of two independent experiments at 15°C. More than 200 animals of each strain were assayed. We attempted dauer counts at 20°C, but daf-2, daf-2 unc-64, and daf-2; unc-31 all formed a high percentage of dauers, making interpretation of synergy difficult. 

^†Worms were grown at 15°C until they were at the L4 or young adult stage and then were shifted to 20°C. Results are the mean ± SD from a single experiment. The number in parentheses is the number assayed. 

^‡Old daf-2; unc-31 animals carry hatched but apparently dead L1 larvae in their body. This does not kill the worms immediately, but it could reduce life span and makes it difficult to interpret the life span data for this strain.