Table 3.

unc-64 acts in the nervous system

Relevant genotype	Expression*	Percent of dauers at 26.8 ± 0.2°C†
N2 (wild-type)	–	12  ± 12
unc-64(e246)	–	100  ± 0
unc-64(e246); saEx357‡	Neurons	70  ± 20
unc-64(e246); saEx358‡	Intestine	99  ± 1

^*Predominant tissue in which the transgene is expressed as determined by fusion of gfp to the identical promoter (data not shown). saEx357 fuses unc-64 to the aex-3 promoter, which is expressed strongly in all neurons and weakly in intestine. saEx358 fuses unc-64 to the elt-2 promoter, which is expressed in the intestine and parts of the pharynx. 

^†Results are the mean ± SD of five independent experiments at temperatures ranging between 26.6 and 27°C. Each experiment had at least 50 animals of each strain. 

^‡These strains carry a chromosomal lin-15(n765) mutation and lin-15(+) in the extrachromosomal array.