Figure 1.

Expression of RuvC in transgenic plants. (A) The expression vector contains the RuvC ORF translationally fused in the carboxyl-terminal region to the NLS derived from the tobacco etch virus (23), and cloned between the cauliflower 35S promoter (35S pro) and the transcription termination region from the octopine synthase gene (Term). This expression cassette was cloned into the pPZP111 binary vector (24), giving rise to clone pGS023, and was transformed in tobacco. (B) Transcription of RuvC was determined by Northern blotting of total RNA and hybridization with the RuvC ORF fragment as a probe. An 800-nt transcript was detected in five independent transgenic plants (background of Su/su cv. Xanthi) transformed with pGS023 (lanes c–g), but not in the nontransformed control Su/su Xanthi line (lanes a and b).