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. 1999 Jun 22;96(13):7415–7420. doi: 10.1073/pnas.96.13.7415

Figure 5.

Figure 5

Function and localization of Hxt2p. (A) Strains HY133 (hxtΔ) and PS1332 (gsf2Δ hxtΔ) containing the vector pRS316 or plasmids expressing Hxt2p (pAK145) or Hxt2–GFP (pAK146) were grown in SC − Ura + 5%glycerol/2%ethanol, and 10-fold serial dilutions were spotted onto SC − Ura + 5%glycerol/2%ethanol and SC − Ura + 2%glucose media. The glucose plate was incubated anaerobically. (B) Wild-type (PS350) and gsf2Δ (PS352) strains containing pAK146 were grown in SC − Ura + 4%glucose and shifted to SC − Ura + 0.1%glucose to induce Hxt2–GFP expression. Cells were harvested 90 min after the shift to low glucose and were prepared for fluorescence microscopy as described in Fig. 2. Exposure times were 4 sec.