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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mol Pharmacol. 2007 Oct 19;73(1):260–269. doi: 10.1124/mol.107.041764

Fig. 5.

Fig. 5

Expression of human HMGB1 restores phosphorylation of p53 and H2ax in Hmgb1 knockout MEFs. Panel A: Accumulation of S18-phsosphorylated p53 and γ-H2ax in Hmgb1−/− cells transfected with human HMGB1-expressing plasmid and treated with 10 μM MP for 28 hr. Expression of HMGB1 in Hmgb1−/− MEFs is shown on the top strip; β-actin, loading control. Panel B: Relative levels of phosphorylation of p53 at Ser 18 and H2ax at Ser 139 after MP treatment of mock- and HMGB1-transfected cells. β-actin was used for normalization of signals.