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. 1999 Jun 22;96(13):7427–7432. doi: 10.1073/pnas.96.13.7427

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Copyright © 1999, The National Academy of Sciences

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Morphological comparison of daf-18, daf-2, and daf-2; daf-18 strains. (Left) Nomarski micrographs. The head of the animal is shown. The two bulbs of the pharynx are indicated by the arrowheads. (Right) Brightfield micrographs. Transgenic animals, shown in the Bottom two rows, carry an extrachromosomal array of either the functional daf-18 gene (Ex[daf-18(+)]), or a mutant derivative that removes exons I and II (Ex[daf-18(−)]). The animals with genotypes daf-2(e1370) and daf-2(e1370); daf-18(nr2037); Ex[daf-18(+)] are dauer larvae and appear thinner in the brightfield micrographs and have a constricted pharynx as shown in the Nomarski micrographs. Wild-type animals and animals with genotypes daf-18(nr2037), daf-2(e1370); daf-18(nr2037) and daf-2(e1370); daf-18(nr2037); Ex[daf-18(-)] are non-dauer L3 larvae.