Table 3.
Effect of the daf-18(nr2037) mutation on adult lifespans
| Strain | Lifespan, days
|
|||
|---|---|---|---|---|
| Mean (±SE) | Ratio vs. wildtype | Maximum | n | |
| 25°C* | ||||
| Wild type | 8.5 (±0.3) | 1.0 | 14 | 34 |
| daf-18(nr2037) | 6.2 (±0.2) | 0.7 | 9 | 48 |
| daf-2(e1370) | 18.8 (±0.5) | 2.2 | 31 | 49 |
| daf-2(e1370); daf-18(nr2037) | 8.3 (±0.4) | 1.0 | 18 | 49 |
| 20°C† | ||||
| Wild type | 12.7 (±0.3) | 1.0 | 22 | 50 |
| daf-18(nr2037) | 5.8 (±0.5) | 0.5 | 15 | 42 |
| daf-2(e1370) | 25.3 (±0.5) | 2.0 | 42 | 50 |
| daf-2(e1370); daf-18(nr2037) | 11.1 (±0.5) | 0.9 | 21 | 50 |
Values are calculated using the data from the experiments shown in Fig. 3. The relative ratio of the mean over that of the wild-type (Bristol N2) strain is also shown. All experiments were performed at least two times with similar results obtained. n, total number of animals scored.
*
The animals were raised at 15°C until the L4 or adult stage and then shifted to 25°C. The day of the shift is counted as day 0 in the adult lifespan assay.
†
The animals were raised and maintained at 20°C. Young adults or L4s were transferred to new plates to assay for lifespan. The day of the transfer is counted as day 0 in the adult lifespan assay.