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Schematic representation of CTV, CTV-ΔCla, and CTV-ΔCla-3′NTR and their replication in N. benthamiana protoplasts. Accumulation of positive- (Left) and negative-stranded (Right) RNAs at 2 and 3 dpi of protoplasts with in vitro transcripts from pCTV-ΔCla (B) or pCTV-ΔCla-3′NTR (C). The position of genomic RNA and sgRNA (promoted by ORF 2 sgRNA promoter) are indicated by arrows and arrow heads, respectively.
